Alok Das
Alok DasChemistry · Research
PRIMARY PROFESSIONAL DISCIPLINE

Chemistry Profile & Research

I am currently pursuing a Master of Science in Chemistry at Rabindranath Tagore University, Assam. My training encompasses qualitative and quantitative chemical analysis, natural product phytochemical extraction, and rigorous laboratory standard operating procedures.

Academic Qualifications

ONGOING POSTGRADUATE
2025 – Present

Master of Science (M.Sc.) in Chemistry

Rabindranath Tagore University, Hojai, Assam

  • Advanced organic, inorganic, and physical chemistry coursework
  • Specialized laboratory training and scientific literature review
  • Focus on analytical instrumentation and research methodologies
GRADUATED DEGREE
2022 – 2025

Bachelor of Science (B.Sc.) in Chemistry

Rabindranath Tagore University, Hojai, Assam

  • Final semester dissertation on Phytochemical Screening of Medicinal Plants
  • Core training in qualitative and quantitative wet chemical analysis
  • Solid foundation in volumetric analysis, titrations, and chemical synthesis

Dissertation Research Project

B.Sc. 6th Semester Project Work Report (Chemistry)
2025 · Rabindranath Tagore University, Hojai, Assam

A Study on Frequently Used Medicinal Plant Leaves in the Village Area of Jamuhandal of Hojai District and Their Phytochemical Analysis

A field-based survey (April–June 2025) of five medicinal plants commonly used by residents of Jamuhandal village, Hojai district, Assam — Neem, Lemon, Sajona (Moringa), Creeping Woodsorrel, and Thankuni (Centella asiatica) — followed by qualitative phytochemical screening of their aqueous leaf extracts.

Problem & Scientific Objective

Rural communities in North-East India, including Jamuhandal, rely heavily on locally available medicinal plants for primary healthcare, yet the specific phytochemical constituents responsible for their therapeutic properties are often undocumented for these particular local specimens.

Analytical Methodology

  • 1Ethnobotanical field survey (April–June 2025) with local residents and traditional healers to identify frequently used medicinal plants; herbarium preparation and identification with the Department of Botany, RTU.
  • 2Leaves washed, shade-dried under pressure for 2 weeks, and ground to a powder using a mortar/electric blender.
  • 3Aqueous extraction: 5 g of powdered sample soaked in 100 ml distilled water for 72 hours, filtered through Whatman filter paper, then centrifuged to obtain a clear extract.
  • 4Qualitative phytochemical screening of each extract: chloroform/conc. H2SO4 test for steroids, 1% lead acetate test for tannins, froth test for saponins, dilute NH3/conc. H2SO4 test for flavonoids, and Wagner's reagent test for alkaloids.

Key Findings & Outcomes

Observation 1

Steroids and saponins were present in the aqueous extracts of all five plants (Neem, Lemon, Sajona, Creeping Woodsorrel, Thankuni).

Observation 2

Tannins were detected only in Neem; flavonoids were present in Neem, Creeping Woodsorrel, and Thankuni, but absent in Lemon and Sajona.

Observation 3

Alkaloids were absent (no red precipitate with Wagner's reagent) across all five plant samples tested.

Ethnobotanical Field Survey
Herbarium Preparation
Aqueous Extraction
Qualitative Phytochemical Screening
Laboratory Documentation & Reporting
Evaluation: Defended with Distinction

Categorized Laboratory & Analytical Skills

Analytical & Wet Chemical Techniques

Core analytical methodologies for compound identification, concentration measurement, and separation.

Qualitative & Quantitative Chemical AnalysisCore

Systematic cation/anion analysis, elemental tests, and gravimetric estimations.

Volumetric TitrationsCore

Acid-base, complexometric, and redox titrations with indicator/potentiometric endpoints.

Thin Layer Chromatography (TLC)Core

Rf value determination, solvent system optimization, and spot visualization under UV/iodine.

Column ChromatographyProficient

Separation and purification of synthetic and natural product mixtures.

UV-Visible SpectrophotometryProficient

Beer-Lambert law applications, absorbance measurement, and calibration curves.

Laboratory Operations & Safety

Hands-on bench experience, solution preparation, equipment handling, and safety protocol adherence.

Reagent Preparation & StandardizationCore

Accurate preparation of molar, normal, and buffer solutions with primary standards.

Serial Dilution & PipettingCore

Precision volumetric dilution handling with calibrated glassware.

Solvent Extraction & RefluxingCore

Liquid-liquid extraction, Soxhlet operation, and continuous reflux setups.

GLP & SOP AdherenceCore

Adherence to Good Laboratory Practice principles and standard operating protocols.

Chemical Hazard & MSDS SafetyProficient

Safe storage, chemical compatibility checks, and spill management protocols.

Quality Control & Documentation

Structured record-keeping, batch testing awareness, data reproducibility, and report preparation.

Scientific Report WritingCore

Preparation of formal dissertation reports, experimental summaries, and findings.

Data Recording & Error AnalysisCore

Experimental variance tracking, standard deviation, and recovery percentage calculations.

QC Calibration RecordsProficient

Maintaining calibration logs for digital balances, pH meters, and laboratory glassware.

M.Sc. Coursework & Academic Focus

Organic Reaction Mechanisms

Nucleophilic substitutions, eliminations, electrophilic aromatic additions, pericyclic reactions, and stereochemical pathways.

Coordination & Transition Chemistry

Crystal field theory, ligand field effects, spectral transitions, and bioinorganic catalytic complexes.

Physical Chemistry & Thermodynamics

Reaction kinetics, chemical equilibria, phase rule, electrochemistry, and spectroscopic state transitions.

Spectroscopic Interpretation

Structure elucidation using combined analytical data: UV-Vis, fundamental IR absorption bands, and basic 1H-NMR principles.

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