Alok Das
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Phytochemical Screening of Medicinal Plant Leaves in Jamuhandal, Hojai

6th Semester B.Sc. Chemistry Project Work Report

Role

Lead Student Researcher

Year

2025

Category

Chemistry & Research

Status

Completed & Verified

Executive Summary & Key Findings

Field survey and qualitative phytochemical screening of five medicinal plants used by residents of Jamuhandal village, Hojai district, Assam — testing aqueous leaf extracts for steroids, tannins, saponins, flavonoids, and alkaloids.

Primary Outcome: Submitted dissertation (23.06.2025) under supervision of Dr. Sujit Ranjan Acharjee, RTU Department of Chemistry; confirmed steroids and saponins in all five plants, tannins in Neem only, flavonoids in three of five plants, and alkaloids absent across all samples.

The Problem & Core Challenge

Rural households in Jamuhandal rely on locally available plants for primary healthcare, but the specific phytochemical constituents behind their traditional medicinal uses were undocumented for these local specimens.

Methodological Approach & Solution

Conducted an ethnobotanical field survey to identify frequently used plants, prepared herbarium specimens, and carried out aqueous extraction followed by standard qualitative reagent tests (chloroform/H2SO4 for steroids, lead acetate for tannins, froth test for saponins, NH3/H2SO4 for flavonoids, Wagner's reagent for alkaloids).

Applied Technologies & Standards

Analytical ChemistryAqueous ExtractionQualitative Phytochemical TestsHerbarium PreparationEthnobotanical Survey

Measurable Outcome & Defense

Submitted dissertation (23.06.2025) under supervision of Dr. Sujit Ranjan Acharjee, RTU Department of Chemistry; confirmed steroids and saponins in all five plants, tannins in Neem only, flavonoids in three of five plants, and alkaloids absent across all samples.

Author: Alok Das·Academic Standing: M.Sc. Candidate·Verification: Documented Monograph / Code Repository

Related Technical Article

Technical Deep Dive

Standard Phytochemical Screening Protocols for Secondary Metabolites

Read the comprehensive methodology and architectural breakdown in the blog.

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